In biomaterial research is important to investigate how different material properties influence cell behavior. Cell-biomaterial interaction is referred to here as the phenomenon involved in adherent cells attachment to the biomaterial surface, and their related cell functions such as adhesion, growth and differentiation. In this context, material surface properties influence the distribution and expression of the adsorbed protein compounds of extracellular matrix, which in turn regulates how cells respond to the material. Understanding the relationship between material surface properties, three-dimensional structure, adsorbed molecules and cellular responses is essential to designing optimal biomaterial able to mimic the physico-chemical, morphological and mechanical properties of tissue to regenerate.
The aim is to investigate the biological material properties in terms of cytotoxicity, biocompatibility and bioactivity by using several cell lines and primary cell cultures (differentiated or mesenchymal stem cells) isolated from different tissues in static and dynamic conditions. In fact, it has been demonstrated that calcium phosphates scaffolds with human mesenchymal stem cells (hBMSC) stimulated by dynamic compression (Bose bioreactor) have shown higher biological activity in terms of vitality, proliferation and extracellular matrix production than a static cell culture. The dynamic tensile stimulation of cellular construct allows an improvement of tendons regeneration than static conditions. Furthermore d dynamic-magnetic stimulation is important to improve the cellular proliferation.
The in vitro studies that we are performing performed to evaluate cell-material interactions are reported below:

  • Adhesion, Vitality and Proliferation
    light microscopy, proliferation test, LDH assay, Alamar blue assay, live/dead staining
  • cell-cell and cell-material interactions

Immunofluorescence, Confocal microscopy, Scanning Electron Microscopy (SEM)

  • Differentiation, protein expression
    Immunofluorescence, ELISA, colorimetric and fluorimetric assays


Raucci MG, Giugliano D, Alvarez-Perez MA, Ambrosio L. Effects on growth and osteogenic differentiation of mesenchymal stem cells by the strontium-added sol–gel hydroxyapatite gel materials. J Mater Sci: Mater Med 2015;26:90

Raucci MG, Alvarez-Perez MA, Meikle S, Ambrosio L, Santin M. Poly(epsilon-Lysine) dendrons tethered with phosphoserine increase mesenchymal stem cell differentiation potential of calcium phosphate gels. Tissue Eng Part A 2014;20(3-4):474-85. DOI: 10.1089/ten.TEA.2012.0450. 

Raucci MG, D’Antò V, Guarino V, Martina S, Valletta R, Ambrosio L. Behaviour of human mesenchymal stem cells on chemically synthesized HA–PCL scaffolds for hard tissue regeneration. J Tissue Eng Regen Med 2013. DOI: 10.1002/term.1768.

Raucci MG, Alvarez-Perez MA, Demitri C, Sannino A, Ambrosio L. Proliferation and osteoblastic differentiation of hMSCs on cellulose-based hydrogels. J Appl Biomater Function Mater 2012;10(3):302-307.

D’Antò V, Martina S, Raucci MG, Ambrosio L, Valetta R. Mesenchymal stem cell proliferation on composite scaffolds for bone regeneration. Eur J Orthodontics 2011;33(6):e67-e68. ISSN 1460-2210.

Raucci MG, D’Antò V, Guarino V, Sardella E, Zeppetelli S, Favia P, Ambrosio L. Biomineralized porous composite scaffolds prepared by chemical synthesis for bone tissue regeneration. Acta Biomater 2010;6:4090-4099.

Raucci MG, D’Antò V, Guarino V, Zeppetelli S, Ambrosio L. Biocompatibility and osteoconductivity studies on hydroxyapatite-polymer composite scaffolds prepared by chemical synthesis. Journal of Applied Biomaterials and Biomechanics 2010;8:123.

Raucci MG, Adesanya K, Di Silvio L, Catauro M, Ambrosio L. The biocompatibility of Silver-containing Na2OCaO2SiO2 glass prepared by sol-gel method. J Biomed Mater Res B Appl Biomater 2010;92(1):102-110.

D’Antò V, Raucci MG, Valletta R, Martina R, Ambrosio L. Fabrication and biocompatibility of composite scaffolds for bone tissue engineering. Eur J Orthodontics 2010;32(6):e7-e8. ISSN 1460-221.

Lee J, Guarino V, Gloria A, Ambrosio L, Tae G, Kim YH, Jung Y, Kim SH. Regeneration of Achilles’ tendon: the role of dynamic stimulation for enhanced cell proliferation and mechanical properties. J Biomater Sci Polym Ed 2010;21:1173-90.



TissueNet, MUR-FIRB RBPR05RSM2. 2007-2011.

REPAIR (PON01-02342) 2011-2015.

MERIT (FIRB-RBNE08HM7T) 2011-2016.

Progetto Invecchiamento PNR-jhost Aging program 2012-2014.



Luigi Ambrosio, Ugo D'Amora, Roberto De Santis, Antonio Gloria, Vincenzo Guarino, Maria Grazia Raucci, Alfredo Ronca, Alessandra Soriente, Stefania Zeppetelli.

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